Bioremediation of drilling wastes contaminated with petroleum using biodemulsifier bacteria and sugarcane bagasse in Khuzestan province

Today, oil pollution is considered one of the soil problems in the oil-rich areas of Khuzestan. One of the effective methods for cleaning petroleum hydrocarbons is "biorefining". This research was carried out in the excavation site ۴۷۹ Maron in ۱۴۰۱. After taking samples from old oil residues, isolation, screening and enrichment of suspension-breaking bacteria (biodemulsifier producer) that have the ability to bind to petroleum hydrocarbons, and bacteria with the best ability to bind to carbon and alkali-loving, including two types of Bacillus and Pseudomonas were selected. After the inoculation of bacteria in the treatments, a comprehensive microbial count and changes in carbon, nitrogen and petroleum hydrocarbons were performed. The results showed a strong reduction in the amount of TPH and C\N ratio in the treatments. In the discussion of heavy metals, the results indicate the absence or very low amount of these elements compared to the standard indicators. The most important limitation of these soils is their high salinity after refining. It is necessary to wash these soils.

Keywords:

Drilling waste , Bioremediation , C/N ratio , TPH , Heavy metals.