The Effect of Aerobic Training and L- Carnitine on BCL۲ and Mitochondrial Enzymes of Rat's Kidney

Objective: Diabetes causes free radical production, oxidative stress and alterations in mitochondrial enzymes and apoptosis. The purpose of this study was to investigate the effect of aerobic exercise and consumption of L-carnitine on BCL۲ and some mitochondrial enzymes of the kidney in diabetic rats. Materials and Methods: In this experimental study, ۴۵ male wistar rats (۲۰۰-۳۰۰ gr) were simple randomly divided into six groups: ۱) placebo group, ۲) healthy control group, ۳) diabetic control group, ۴) diabetic group receiving L- carnitine, ۵) diabetic group of aerobic training and ۶) diabetic group of aerobic training and recipient of L-carnitine. Rats with a serum glucose level higher than ۳۰۰ mg/ dL were considered diabetic. L-carnitine recipients received ۱۰۰ mg of L-carnitine daily orally. The dependent variables of the study were measured ۲۴ hours after the last training program session by ELISA in kidney tissue. Data were analyzed by Shapiro- Wilk and two way ANOVA and Tukey post hoc test at P-value< ۰.۰۵. Results: The results showed that the combined effect of aerobic training and supplementation of L-carnitine on Bcl۲ factor of kidney tissue of rats with diabetes has a significant effect (P-value: ۰.۰۱۹). But aerobic exercise (P-value: ۰.۹۶۹) and supplementation of L-carnitine (P-value: ۰.۵۸۴) were not significant. Conclusion: The results of this study showed that the combined effect of aerobic exercise and supplementation of L-carnitine on CPT۲ and Malonyl COa and Bcl۲ have a significant effect on the kidney of diabetic rats. Also, exercise alone and consumption of L-carnitine alone do not have a significant effect.

Keywords:

Aerobic training , L-carnitine , Diabetes , CPT۲ , Malonyl CO A , Bcl۲ , Kidney.