Study of Potential Probiotic Properties of Lactic Acid Bacteria Isolated from Raw and Traditional Fermented Camel Milk

In the present research work, the potential probiotic properties of Lactococcus lactis KMCM۳ and Lactobacillus helveticus KMCH۱ isolated from raw camel milk and traditional fermented camel milk (Chal), respectively, were studied. The probiotic properties of isolates that were investigated included the hemolysis, antibiotic resistance, antibacterial features, resistance to low pH and bile salts, survival under simulated GastroIntestinal Tract (GIT) conditions, adhesion ability to hydrocarbon, and their auto-aggregation and co-aggregation rates. None of isolates exhibited hemolytic activity. They were susceptible against tetracycline, penicillin, ampicillin, chloramphenicol, erythromycin and vancomycin. Lac. lactis KMCM۳ and L. helveticus KMCH۱ retained their viability at pH ۳.۰ (۸.۶۸ and ۸.۶ log cfu mL-۱, respectively), ۰.۳% w/v bile salts (۸.۲۳ and ۸.۵۸ log cfu mL-۱, respectively) and under simulated GIT conditions (۸.۳۱ and ۸.۴۶ log cfu mL-۱, respectively). Both of these isolates inhibited the growth of E. coli, S. aureus, L. monocytogenes, B. cereus and S. enterica subsp. enterica serovar Typhimurium with MIC values of ۶.۲۵ to ۲۵ mg mL-۱. In addition, They exhibited an ability to adhere to hydrocarbon (xylene), and possessed a high auto-aggregation and co-aggregation rate (more than ۴۰%).

Keywords:

Lactococcus lactis , Lactobacillus helveticus , Chal , Auto-aggregation rate , Co-aggregation rate.