Identification of Immunogenic Proteins in Early and Advanced Stages of Breast Cancer: An Immunoproteomics Study
Publish place: Middle East Journal of Cancer، Vol: 14، Issue: 4
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
View: 75
This Paper With 11 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_MISJ-14-4_003
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
Background: Early detection of breast cancer (BC) is extremely important as late diagnosis has been associated with a high rate of mortality. Immunogenic proteins and autoantibodies have been considered as favorable targets for early detection and targeted therapy in cancer. Accordingly, the present study aimed to identify the immunogenic antigens in both early and advanced stages of BC via a serologic proteome analysis (SERPA) approach.Method: This is a case-control study wherein we separated the proteins from BC tissues in the early stages (n = ۱۰) and advanced stages (n = ۱۰) utilizing two-dimensional electrophoresis (۲DE) and then transferred them onto a Polyvinylidene Difluoride (PVDF) membrane. To explore the tumor antigens reacting with antibodies, two-dimensional (۲D) blots of tumor tissues in the early and advanced stages were separately probed with the sera from the same patients. Afterwards, we identified antibody-reactive proteins via liquid chromatography with tandem mass spectrometry (LC-MS/MS).Results: Fibrinogen beta chain (FGB), protein deglycase DJ-۱(PARK۷), and peroxiredoxin-۲ (PRDX۲) were the highly reactive antigens identified in the earlystage patients. In addition, RuvB-like۱ (RUVBL۱) and triose phosphate isomerase (TPI) were recognized as the immune reactive proteins in the late-stage patients.Conclusion: The results herein revealed that the immune-proteome pattern of BC patients changes along with tumor progression from primary to advanced stages. Moreover, immunogenic proteins seemed to stimulate the humoral immune system to produce autoantibodies in the initiation phase of BC; these autoantibodies could be employed as complementary factors for early detection of BC. The findings are however preliminary, and further studies with a larger sample size are required for verification and validation of previous findings.
Keywords:
Authors
Zeinab Mohammadi
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Mahdi Pouramir
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Mohammad Reza Haghshenas
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Sedigheh Tahmasebi
Breast Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abbas Ghaderi
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :