Rs۱۲۹۴۵۴۱۸۶۱ modulates Endocytosis signalingpathway in gastric cancer patients via change in thebinding affinity of miR-۱۳۳a-۵p to MFSD۴A

Introduction: Gastric cancer is a disease in which malignant(cancer) cells form in the lining of the stomach. Age, diet, andstomach disease can affect the risk of developing gastric cancer.Gastric cancer consists of two pathological variants, intestinaland diffuse. The intestinal-type is the end-result of an inflammatoryprocess that progresses from chronic gastritis to atrophicgastritis and finally to intestinal metaplasia and dysplasia.Fortunately, dedicated research into its pathogenesis and identificationof new risk factors, treatment, and advanced endoscopictechniques have led to earlier detection of gastric cancer.Materials and Methods: First of all, GSE۵۴۱۲۹ raw data wasselected from Gene Expression Omnibus (GEO) and analyze byR studio to obtain differentially expressed genes(DEGs). Thelimma package was used for the statistical analyses and findingthe novel DEGs. Protein-protein interaction analysis wasperformed by STRING online software. Pathway enrichmentanalysis was performed by ENRICHR. Single nucleotide polymorphism(SNP) analysis was performed by miRNASNP .v۳Results: Based on the microarray analysis۱, MFSD۴A (logFC:۱.۵۷, adj. P. Val: ۰.۰۴) has a significant uo-regulation in theretinoblastoma patients, compared to control. HIAT۱, SVOP,SCNN۱A, CAMSAP۱, CBY۱ has a significant protein interactionwith MFSD۴A. Based on the pathway enrichment analysis,MFSD۴A and its interactome has a significant contribution inthe regulation of Endocytosis and Pathways of neurodegenerationsignaling pathway. miRNASNP analysis revealed thatrs۱۲۹۴۵۴۱۸۶۱(G/C) regulates the expression level of MFSD۴Aby reducing the binding affinity of hsa-miR-۱۳۳a-۵p miRNAto this mRNA.Conclusion: Rs۱۲۹۴۵۴۱۸۶۱ has a significant novel role inthe modulating the Endocytosis and Pathways of neurodegenerationsignaling pathway by regulating the expression levelof MFSD۴A in the retinoblastoma patients. Mentioned regionof DNA could be a novel therapeutic target for the prognosis,diagnosis, and possible treatment strategies for retinoblastomapatients.

Keywords:

Gastric cancer , System Biology , Microarray analysis , RNA Interaction , Signal Nucleotide polymorphism