Transcription Factor Assay of Peripheral Blood T cells in Different Groups of Rheumatoid Arthritis Patients

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نوع سند: مقاله ژورنالی
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JR_REMJ-8-4_001

تاریخ نمایه سازی: 20 دی 1402

Abstract:

Background: Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation of the joints and other tissues and organs of the body. Previous reports have demonstrated the imbalance of T helper (Th) subsets and Treg activity in the development, progression, and remission of RA. Here, we investigated the mRNA expression of four major transcription factors T-bet (Th۱), GATA (Th۲), RORc (Th۱۷), and Foxp۳ (Treg) in peripheral blood of different groups of RA patients. Materials and methods: In this case-control study, ۶۰ patients with RA, including ۲۰ newly diagnosed, ۲۰ under treatment, and ۲۰ in remission, as well as ۲۰ patients with osteoarthritis, and ۲۰ age- and the sex-matched healthy individual were enrolled. Diagnosis and classification of patients were done according to the American College of Rheumatology criteria. The relative mRNA expression of transcription factors, including T-bet, GATA, RORc, and Foxp۳, was measured using qRT-PCR. Results: The relative expression of T-bet in RA patients was significantly increased in healthy controls (P = ۰.۰۰۲), while the relative expression of Foxp۳ in RA patients was significantly decreased in healthy controls (P < ۰.۰۰۰۱). There was no significant difference in the expression of GATA۳ or RORc among RA patients, healthy controls, and osteoarthritis group. Conclusions: The results indicate the importance of Th۱ and Treg cells in RA; however, the role of Th۱۷ cells appear to be of little importance in these patients. It seems that Th۲ cells do not interfere with RA development.

Authors

Saeid Taghiloo

Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Abolghasem Ajami

Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Mohsen Tehrani

Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Arezou Abbasi

Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Reza Alizadeh-Navaei

Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran

Mohsen Akhiani

Department of Rheumatology, Alborz Hospital, Alborz University of Medical Sciences, Karaj, Iran

Alireza Salami

Department of Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

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