Evaluation of antibacterial activity of ozonated oil and ozonated water against Neisseria gonorrhoeae and Neisseria meningitidis by broth microdilution method

The necessity of using ozonated oil and water for treating infections such as gonorrhea and meningitis which their treatment by common antibiotics is sometimes difficult and it is becoming more apparent every day.  In this study, the antibacterial effect of ozonated oil and water against Neisseria gonorrhoeae (N. gonorrhoeae) and N. meningitidis were investigated using Broth microdilution methods at zero, ۲۴ and ۴۸ hours after incubation at ۳۷°C. The results were determined by culturing bacteria on their specific culture medium and using an ELISA reader to determine minimum inhibitory and bactericidal concentrations (MIC and MBC, respectively). The results showed that ozonated oil with PI=۵۰۰ had an expressive effect on N. meningitidis. The concentration of ۰.۰۹PI was determined as MIC and the concentration of ۰.۱۹PI was determined as MBC. The ozonated oil with PI=۱۰۰۰ had an expressive effect on N. gonorrhoeae. The concentration of ۳.۱۲ PI was determined as MIC and the concentration of ۶.۲۵ PI was determined as MBC. Ozonated water containing ۲.۵ mg/L of ozone had no significant antimicrobial effect on the studied bacterial species. The results of this study showed that ozonation of oil may improve its chemical properties. In addition, by increasing the incubation time to ۲۴ hours, ozonated oil showed a favorable antibacterial effect against N. gonorrhoeae and N. meningitidis.

Keywords:

Gram-Negative Bacteria , Meningococcus , Neisseria gonorrhoeae , Neisseria meningitides , Ozone therapy