Molecular Characterizations and Antimicrobial Susceptibility of Extended-Spectrum ß-lactamase (ESBL) Producing Proteus spp. Clinical Isolates in Babol, Northern Iran

Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
View: 58

This Paper With 8 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_JMMI-10-3_003

تاریخ نمایه سازی: 29 بهمن 1402

Abstract:

Introduction: Proteus spp. are opportunistic members of Enterobacteriaceae, accounting for ۱۰% of urinary tract infections and other primary clinical infections. They produce extended-spectrum beta-lactamases (ESBL) that can confer resistance to beta-lactam antibiotics. This study aimed to investigate the prevalence, antimicrobial susceptibility, molecular characteristics, and genetic relationship of ESBL-producing Proteus spp. clinical isolates in Babol, Northern Iran. Methods: In this cross-sectional study, out of ۱۱۲ clinical samples, ۳۰ Proteus spp. isolates were identified via specific biochemical assays. According to the Clinical and Laboratory Standards Institute (CLSI) guidelines, antibiotic susceptibility was evaluated using disc diffusion and agar dilution methods, and polymerase chain reaction (PCR) was used to detect blaTEM and blaSHV genes. Results: The resistance rate to tetracycline and sulfamethoxazole was highest by disk diffusion and agar dilution. Multiple drug-resistant (MDR) isolates were ۸۶% and ۶۰% in disk diffusion and agar dilution assays. Seven (۲۳.۳%) isolates had the blaTEM genes and ۱۸ (۶۰%) blaSHV. Conclusion:  ESBL-producing Proteus spp. was highly prevalent, and the blaSHV was the most common resistance contributing gene. These findings and relatively high resistance to ampicillin demand more care in prescribing antibiotics. Also, the high prevalence of MDR isolates in patients infected with ESBL-producing Proteus spp. requires continuous surveillance.

Authors

Atena Malaki

Department of Biotechnology, Islamic Azad University, Damghan, Iran

Elaheh Ferdosi-Shahandashti

Department of Medical Biotechnology, School of Medicine, Babol University of Medical Sciences, Babol, Iran; Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran

Amirhosein Maali

Department of Immunology, Pasteur Institute of Iran, Tehran, Iran

Parisa Sabbagh

Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran

Aynaz Khademian

Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Malekjamshidi MR, Shahcheraghi F, Feizabadi MM. Detection and PFGE analysis ...
  • Peirano G, Pitout JD. Extended-spectrum β-lactamase-producing Enterobacteriaceae: update on molecular ...
  • Ramadan AA, Abdelaziz NA, Amin MA, Aziz RK. Novel blaCTX-M ...
  • Palmeira JD, Ferreira HMN. Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle ...
  • Cullik A, Pfeifer Y, Prager R, Baum Hv, Witte W. ...
  • Gröbner S, Linke D, Schütz W, Fladerer C, Madlung J, ...
  • Knudsen PK, Brandtzaeg P, Høiby EA, Bohlin J, Samuelsen Ø, ...
  • Tumbarello M, Trecarichi EM, Fiori B, Losito AR, D'Inzeo T, ...
  • Vivas R, Barbosa AAT, Dolabela SS, Jain S. Multidrug-resistant bacteria ...
  • Drzewiecka D. Significance and roles of Proteus spp. bacteria in ...
  • Armbruster CE, Forsyth-DeOrnellas V, Johnson AO, Smith SN, Zhao L, ...
  • Schaffer JN, Pearson MMJ. Proteus mirabilis and urinary tract infections. ...
  • Chen C-Y, Chen Y-H, Lu P-L, Lin W-R, Chen T-C, ...
  • Avlami A, Bekris S, Ganteris G, Kraniotaki E, Malamou-Lada E, ...
  • Japoni S, Japoni A, Farshad S, Ali AA, Jamalidoust M. ...
  • Stalder T, Barraud O, Casellas M, Dagot C, Ploy M-C. ...
  • Chen L, Al Laham N, Chavda KD, Mediavilla JR, Jacobs ...
  • Uzunović S, Ibrahimagić A, Hodžić D, Bedenić B. Molecular epidemiology ...
  • Fattah Hamid S, Bahadeen Taha A, Jamel Abdulwahid M. Distribution ...
  • Li Z, Peng C, Zhang G, Shen Y, Zhang Y, ...
  • Di Conza JA, Badaracco A, Ayala J, Rodríguez C, Famiglietti ...
  • Chinnam BK, Nelapati S, Tumati SR, Bobbadi S, Chaitanya Peddada ...
  • Algammal AM, Hashem HR, Alfifi KJ, Hetta HF, Sheraba NS, ...
  • Malekjamshidi MR, Shahcheraghi F, Feizabadi MM. Detection and PFGE analysis ...
  • Fouch S, Mitchell J, Lwaleed B, Zinkevich VJ. Evaluation of ...
  • Liakopoulos A, Mevius D, Ceccarelli DJ. A review of SHV ...
  • Fattahi K, Rostamzad AJ. Distribution of blaCTX-M, blaTEM genes among ...
  • Uyanga FZ, Ekundayo EO, Nwankwo EOJ. bla TEM, bla SHV ...
  • Lev AI, Astashkin EI, Kislichkina AA, Solovieva EV, Kombarova TI, ...
  • Musa HA, Osman MA, Abdelaziz YH, Mohamed S, Ibrahim-Saeed MJ. ...
  • Kurihara Y, Hitomi S, Oishi T, Kondo T, Ebihara T, ...
  • Schmiedel J, Falgenhauer L, Domann E, Bauerfeind R, Prenger-Berninghoff E, ...
  • Hujer AM, Page MG, Helfand MS, Yeiser B, Bonomo RA. ...
  • نمایش کامل مراجع