Detection of Virulence Genes of Clostridium difficile in Children with Cancer by Multiplex PCR
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JMMI-2-3_002
تاریخ نمایه سازی: 29 بهمن 1402
Abstract:
Introduction: Toxigenic Clostridium difficile is the major cause of antibiotic-associated diarrhea, colitis, and pseudomembranous colitis. The pathogenicity of C. difficile is related to toxins A&B. Children with cancer are at risk of developing C. difficile infection (CDI) due to increased exposure to antibiotics, immunosuppression, and longer hospital stays. Recently, due to higher sensitivity and specificity of nucleic acid amplification test (NAATs) compared to toxin enzyme immunoassays (EIAs), many laboratories are transitioning to NAATs for detection of CDI. We aimed to use a multiplex PCR to detect the C. difficile toxin genes tcdA, tcdB and tpi in stool of cancerous children. We also aimed to show the effects of chemotherapy regimens on the prevalence of C. difficile in these children. Methods: ۱۰۵ fecal samples were collected from cancerous children who were hospitalized and undergoing chemotherapy. The presence of tcdA, tcdB, and tpi genes were tested. Results: C. difficile was identified in ۱۷.۱۴% of children and the detection rate of A-B+ strains was higher than A+B+ and A+B- strains. C. difficile was found in ۱۷.۸% of children who received single antibiotic (۵/۲۸ cases virulence genes were detected in ۴ cases) and in ۴۱.۴% of patients who received more than one antibiotics (۱۲/۲۹ cases virulence genes were detected in ۸ cases﴿. Conclusion: Multiplex PCR is a powerful technique for preliminary screening and rapid detection of C. difficile and its virulence genes in the stool of cancerous children. The prevalence of C. difficile in cases receiving several antibiotics was higher than those receiving single antibiotics.
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Authors
Hadis Tavafi
Department of Biology, Faculty of Sciences, Shahed University, Tehran, Iran
Parviz Owlia
Molecular Microbiology Research Center, Shahed University, Tehran, Iran
Fariba Shirvani
Pediatric Infections Research Center, Mofid Children Hospital, Tehran, Iran
Mozhgan Hashemie
Pediatric Infections Research Center, Mofid Children Hospital, Tehran, Iran
Nader Shahrokhi
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
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