Bioinformatic and Phylogenetic Investigation of WRKY Genes Involved in Drought Stress in Camelina sativa Plant

Camelina (Camelina Sativa) is a hexaploid dicotyledonous plant from the Brassicaceae family, which is very similar to the Arabidopsis. The number of camelina chromosomes is ۴۰=۲n. WRKY transcription factors are one of the most important gene families in plants that play an important role in regulating growth and development and response to various stresses. In this research, ۲۲۴ WRKY genes were identified in the camelina plant by searching the database, and the chromosomal position, gene length, and conserved motifs were identified in the camelina plant based on the Arabidopsis. Also, in this study, in order to validate the research, ۲ genes WRKY۸ and WRKY۵۷ under drought stress were investigated by the qPCR method. The results indicated that both the above-mentioned genes were strongly expressed under drought stress conditions in tolerant varieties compared to normal conditions, but the trend was opposite in sensitive varieties. This study has provided acceptable and valuable information for studying the evolution and function of the WRKY gene family in camelina.

Keywords:

Abiotic stress , qRT-PCR , Brassicaceae , Transcriptional factors , WRKY