Formulation and prototyping of two types of emergency food ration promotingthe immune system of soldiers and investing the effects of immunity boosting onthe animal model of mice

Background: The rescue and protection of refugees and disaster victims depend on fast fooddelivery at the lowest possible cost. Most of the time, emergency rations are used as the sole sourceof food available early on in natural disasters such as earthquakes, hurricanes, etc., or in times ofemergency such as evacuation of a war zone. The amount of energy, nutrients, sensoryacceptability, and immune-boosting components of emergency rations is of the utmost importanceto meet the nutritional needs of those affected. Nutrition and nutrient-boosting foods are essentialfor proper immune function and function.The aim of this study was to formulate and sample two emergency dietary rations promoting theimmune system of warriors and to investigate its immunogenic effects on the animal model of mice.Methods: In this study, 4 groups of 10 Balb / c mice aged 4-6 weeks and weighing 17.8-18.9 g willbe selected. Two dietary groups received treatment 1 and 2 and two control groups received 1 and 2diets. Before the beginning of the experiment, all rats were kept for a week for adaptation,adaptation and relieving stress in the animal site of Mashhad University of Medical Sciences. Then,the blood samples of all rats were examined for immune factors. Then the diets of treated andcontrol rats were given 3–4 g daily for 8 weeks. During this period health status and water intakeand Mice were examined for food. After 8 weeks of dietary intake, venous blood samples weretaken to evaluate concentrations of IL4, IFN-, IGG1, and IGG2. Humoral is in mice determined byELISA.Results:Results showed that the treatment groups had higher levels of gamma interferon than thecontrol groups. Also, the ratio of IFNy / IL4 in the treated groups was higher than the controlgroups. These results confirm an increase in the Th1 / Th2 cell ratio and an increase in cellularimmune responses in the treatment group. According to these results, IgG2 and IgG1 antibodiesincreased and decreased in the treated group, respectively. It is noted that isotype switching to IgG2and IgG1 in mice is affected by IFNy and IL4, respectively; the ratio of these antibodies isconsistent with the findings of the cytokines assay and the apparent increase in cell ratio. Th1 in thetreatment-receiving group.Conclusion: The use of emergency diet in mice increased the cellular immune responses in bothtreatment groups.

Formulation and prototyping of two types of emergency food ration promotingthe immune system of soldiers and investing the effects of immunity boosting onthe animal model of mice Keywords:

Practical Emergency Diets , ELISA , Mice , Immune System