Characterization of IgY antibodies, developed in hens, directedagainst camel immunoglobulins
Publish place: Iranian Journal of Veterinary Medicine، Vol: 3، Issue: 1
Publish Year: 1388
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJVM-3-1_006
تاریخ نمایه سازی: 26 اردیبهشت 1394
Abstract:
Chicken egg yolk antibodies (IgY) against camel immunoglobulins were generated and their specificity was shown by double immunodiffusion,munoelectrophoresis, and Western blotting. Anti camel antibodies specifically react to camel antibodies in immunodiffusion andimmunoelectrophoresis were demonstrated. Western blotting revealed that IgY antibodies recognize the heavy chain in a number of mammalian species. Anti-camel antibodies bound camel IgG1, IgG2, IgG3 subclasses like the subclasses of sheep, but no reactivity to all subclasses chains in cattle and horse antibodies were observed. Polyclonal anti-camel IgY was specifically light chain reactive. Overall, the potential of using egg yolk immunoglobulins as a convenient source of anti antibodies to camel immunoglobulins was demonstrated
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Authors
Gh Nikbakht Brujeni
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran,Tehran-Iran.
S Tabatabaei
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran,Tehran-Iran.
M Khormali
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran,Tehran-Iran.