Polymerase Chain Reaction for the Diagnosis of Tuberculosis

We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsenstaining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfastbacilli negative paucibacillary forms of TB. However, we intend to highlight few points regarding PCR which may add to practical applicability of the study