Study of protein profile of wheat bread in seedling stageusingtwo- dimensional electrophoresis

Wheat is the first and foremost cereal crop in the world. The interval between the germination and early tillering stage is called seedling. The seedling stage, seed embryo has three initial leaf cells, which after germination, vegetative shoot apical meristem begins to be divided leading to the emergence of the leaves. In this study,proteomics has been used to study changes of protein profiles in wheat leaves in the different stages of seedling before tillering. To investigate changes in protein profiles, samplings took place in three stages: unifoliate ,bifoliate and trifoliate. To study and identify the proteins associated with the seedling, total protein wasextracted from wheat leaf in seedling stages byTCA-ACETON method and separated based ontwo-dimensional electrophoresis method. After analysis of gels obtained from the two-dimensional electrophoresis through Software Image master ۶, ۳۶۰ differentially expressed protein spots (DEPs) were detected. Among which, ۳۱protein spots showed significant changes in the level of ۵%, in various stages of seedling. ۵۵% of the identified proteins showed decreased expression and ۱۹% showed increased expression and ۲۶% of the identified proteins did not indicate significant regulatory changes.

Keywords:

Proteomics , Seedling , Two-dimensional electrophoresis , Wheat