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Histochemical analysis of expression pattern of CaMV 35S promoter in transgenic rapeseed (Brassica napus L.)

Publish place: The Second National Conference on Applied Research in Chemistry, Biology and Geology
Publish Year: 1393
Type: Conference paper
Language: English
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Link to this Paper:
https://civilica.com/doc/452614

Document National Code:

CBGCONF02_276

Index date: 28 March 2016

Histochemical analysis of expression pattern of CaMV 35S promoter in transgenic rapeseed (Brassica napus L.) abstract

Promoters are considered valuable tools for biotechnology and provide great opportunities for eugenic purposes. This study aimed to investigate the expression pattern of GUS gene directed by CaMV 35S promoter in transgenic rapeseed (Brassica napus L.). The GUS gene was transferred to the rapeseed explants by Agrobacterium. The regenerated and rooted transgenic plantlets were transferred to the pots containing a mixture of soli and vermiculite. These plants underwent PCR and histochemical GUS assay. The cross-section of leaf, petiole, and stem of the transformed plants was obtained. GUS activity was observed in phloem, parenchyma, collenchyma, and supporting tissue of vascular bundles. It was also observed in cambium, endoderm, vascular ray, pith parenchyma, epidermis, and trichomes. These results showed that CaMV 35S causes the expression of transgene in various tissues differently.

Histochemical analysis of expression pattern of CaMV 35S promoter in transgenic rapeseed (Brassica napus L.) Keywords:

Vascular Bundle , GUS , Tissue Culture , Transformation , Agrobacterium

Histochemical analysis of expression pattern of CaMV 35S promoter in transgenic rapeseed (Brassica napus L.) authors

Parissa Jonoubi

Department of Plant Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Ali Hatef Salmanian

Department of Plant Biotechnology,National Institute of Genetic Engineering and Biotechnology (2IGEB), Tehran, Iran

Amin Ravaei

Department of Plant Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

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