The study of biochemical parameters of Common Carp (Cyprinus carpio L.) juvenile in transportation using Celmanax liquid Yeast by supplementation in diet

The effects of Celmanax liquid commercial perbioticon biochemical parameters Common Carp (Cyprinus carpio L.) juvenile were studied during a long-distance transportation in plastic bags. 300 Common Carp juvenile with average weight of 30.50±1.50 g were fed by supplemented diet of Celmanax liquid commercial perbioticwith level of 1ml/kg of diet at during of 90 days and introduced to 20 liter plastic bags in stocking density of 0.1 kg/ l in 5 treatments (water temperature of 25oC, 18oC, 10oC, water salinity of 5 and 0.5 g/l) with three replicates. In total 18 plastic bags were used in this trial. The experiment was carried out in completely random design. The effect of 12 h transport period on the biochemical profiles of common carp were investigated in experimental treatments. A quantity of 2-3 mL of blood was taken from 10 fish of plastic bag by caudal venous puncture at the beginning and the ending of the experimental trial. The aspartate aminotransferase (AST) alkaline phosphatase (ALP), alanine aminotransferase (ALT) and activities were estimated using commercial kits (Pars Azmoon, Tehran, Iran) and an Auto analyser. The level of plasma glucose of Common carp juvenile was 198.50 ng/ml in control group and was decreased to 135.00 ng/ml in treatment of A (fish were fed by Celmanax liquid commercial perbiotic supplemented diet) after 12 h transportation period at temperature of 25oC , while the decrease of water temperature to 10 oC , increased this immunity factor to 203.50 ng/ml. The maximum of AST (361.50 IU/L) was obtained in treatment of C where the common carp juvenile were transported for 12 h in water of 10 oC but the minimum (129.5 IU/L) of this liver enzymes in plasma was observe in water of in treatment of A. The transportation of Carp juvenile in control group had lowest ALP (27.07 U/L). However using the Celmanax liquid commercial perbiotic in experimental treatments at transportation of Common Carp in plastic bag had variety effects on plasma biochemical parameters.

Keywords:

Celmanax liquid yeast , Biochemical parameters , transportation , Common Carp , cortisol