Role of INPP5A in progression of esophageal squamous cell carcinoma

The inositol polyphosphate 5-phosphatases are member of signal-terminating enzymeswhich hydrolyzethe five-position phosphate and inactivate the inositol 1,4,5-trisphosphate(Ins(1,4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P. Inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate as a second messenger molecules, playa critical role in calcium release from intracellular stores and initiation of some importantcellular response such as proliferation. Recent studies significantly showed the impact ofinactivation of a second messenger molecules in progress of tumorgenesis function. Toexplore the involvement of INPP5A in tumorigenesis, we assessed the mRNA expressionand protein level of ESCC samples and analyzed the possible correlation with differentclinicopathological features of patients. The mRNA expression of Inpp5A was comparativelyanalyzed by real-time PCR in tumor and related margin normal tissues of 48 ESCC patients.Immunohistochemistry analysis was applied to investigate the level of protein expression inESCC patients. Our result indicated the 30% under expression of Inpp5A in mRNA level. Thelow level of INPP5A protein expression was significantly correlated with increased size oftumor mass (P= 0.013, Correlation coefficient = 0.472), progressed stage of the disease (P=0.019), and lymph node metastasis of tumor cells (P = 0.052). Survival rate of ESCC patientsand outcomes are directly correlated with early detection in lower stages. Our data indicatethat under expression of INPP5A significantly correlated with stage, size and depth of tumor.According our results, loss of activity of INPP5A gene may play an important role inprogression of malignancy and can be considered in therapeutic modalities to restrict ESCCprogression.

Keywords:

Expression of INPP5A , Tumor , Early detection , biomarker , Esophageal Squamous Cell Carcinoma