Nonenzymatic glucose detection based on copper oxide-Palladium/CNT nanocomposite

For diagnosis and management of diabetes, precise and accurate detection of glucose is very important. Therefore, producing high-performance analytical tools for diagnosis of diabetes andmanagement of blood glucose is very crucial. Glucose oxidase based biosensors have been proved to be powerful devices for glucose determination thanks to their high selectivity, sensitivity and low detection limit. However, these biosensors due to the intrinsic feature ofenzymes have some defects in stability and reproducibility. Therefore, the non-enzymatic glucose biosensors based on metal nanoparticles and metal oxides have received more and more attentions. non-enzymatic biosensors have many advantages such as simple preparation, lowcost, high stability and sensitivity. In current study, we combined CuO nanoparticles with Palladium and carbon nanotubes to produce a nanocomposite (CuO-Palladium/CNT) for nonenzymaticdetection of blood glucose. Based on cyclic voltammetric results CuOPalladium/ CNT nanocomposite has a high sensitivity (2000 μA mM-1 cm-2), good linear range (0.002-7 mM), low detection limit (0.2 μM) and short response time, good anti-interference andanti-toxicity, long-term stability (over 8 weeks). Non-enzymatic glucose biosensors like CuOPalladium/ CNT nanocomposite alleviated the problems of enzyme-based biosensors such as insufficient stability, sensitivity to temperature, pH and humidity, lower reproducibility, thermaland chemical instability. Because of these properties and easy preparation method, CuOPalladium/ CNT nanocomposite is a promising material for marketing of glucose biosensors. Also complementary experiments to examine the sensor for detection of glucose in real human serum are considered.

Keywords:

Biosensor , Blood glucose , Non enzymatic nanoparticles , Copper oxide , Diabetes