Effect of follicle stimulating hormone and testosterone on viability rate of cryopreserved spermatogonial stem cell after thawing
Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJVST-5-1_001
تاریخ نمایه سازی: 11 تیر 1396
Abstract:
Stem cells are generally defined as clonogenic cells capable of both self-renewal and differentiation. Probably the best method for long-term preservation of spermatogonial stem cells is cryopreservation. In this study, effects of Follicle Stimulating Hormone and Testosterone on viability rate of cryopreserved spermatogonial stem cell after Thawing were investigated. Sertoli and spermatogonial cells were isolated from 3-5 months old calves. Coculturedsertoli and spermatogonial cells were treated with Follicle Stimulating Hormone and Testosterone in treatment groups before cryopreservation. Results indicated that Follicle Stimulating Hormone increased viability rate of cryopreserved spermatogonial cells in comparison with Testosterone and control group. In conclusion, using Follicle Stimulating Hormone provided proper bovine spermatogonial stem cell culture medium for in vitroculture and cryopreservation of these cells.
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Authors
Reza Narenji Sani
Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Parviz Tajik
Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Mohammad Hasan yousefi
Department of Basic Science, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
Mansoureh Movahedin
Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran