Effects of protein, vitamins, essential amino acids and glucose on growth and proliferation of baby hamster kidney cells (BHK) in suspension culture

The present study focuses on the effects of protein, glucose as a carbohydrate, essential amino acids and vitamins on the baby hamster kidney (BHK) cells growth and proliferation. amino acids and vitamins on the baby hamster kidney (BHK) cells growth and proliferation BHK cells culture is of special importance in pharmaceutical and biological products, particularly animal drugs. BHK cells culture is very important in production processes. All experiments have been done under suspension culture conditions in a 1-liter bioreactor having blades and air mixing pores. Considering the final cell number, which is the most important factor in studying the state of cell culture under suspension conditions, the effect of protein glucose, essential amino acids and vitamins on BHK cells has been investigated. The higher final number of cells means higher cell culture productivity. During the study, it was indicated that the glucose, protein, essential amino acids and vitamins have the greatest impact on BHK cells growth and proliferation, respectively.

Keywords:

supplement , baby hamster kidney (BHK) , suspension , growth , proliferation