Association of Ki67 expression and CK19 detection in breast cancer patients
Publish place: 10th International Breast Cancer Congress
Publish Year: 1393
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ICBCMED10_040
تاریخ نمایه سازی: 21 اردیبهشت 1397
Abstract:
Cytokeratin19 (CK19) is a part of the cytoskeleton which abundantly expressed in the majority of epithelial tumor cells and is used as a tumor marker in cancer. Ki67 is a cellular proliferation marker as well as a cancer antigen that is found in growing and dividing cells. Thischaracteristic makes Ki67 a good tumor marker. Methods: In the present study, 25 patients and 25 healthy women as control were evaluated. Total RNA was extracted from blood. RT-PCR technique was applied to the extracted RNA, using specific primers. RT-PCR assay was used for detection of CK19 tumor marker and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as an internal control.Results: In this study, CK19 was detected in 32% of breast cancer patients but CK19 was not positive in the healthy controls. The CK19 gene expression in blood was assessed and compared with Ki67 gene expression; pathologic characteristic of patients. The association of the CK19 and Ki67 gene expression was statistically significant with P value =0.000. Conclusion: It was shown that CK19 gene expression is potentially related to the Ki67 expression and it could be involved in the pathogenesis of breast cancer.
Authors
Nasrin Karimi
Master of Sciences, Department of Biology, Ashkezar Branch, Islamic Azad University, Ashkezar,Iran
Mana Oloomi
Associate Professor, Department of Molecular Biology, Pasteur Institute of Iran, Tehran-Iran
Mahmood Dehgani Ashkezary
Associate Professor ,Department of Biology, Ashkezar Branch, Islamic Azad University, Ashkezar,Iran
Seyed Mahdi Kalantar
Professor in Molecular Genetics, Yazd Research and Clinical Center for Infertility, Yazd-Iran