Assessment of serum level of obestatin and its relationship with insulin resistance in the PCOs women with normal BMI

Background: Polycystic ovary syndrome is one of the most common endocrine disorders that associated with hyper-androgenism and chronic anovulation, affecting 5-8% of reproductive-age women; also at least 50% of PCOs women have insulin resistance. Obestatin is a peptide hormone that secreted by the cells of stomach and small intestine, also derived from the precursor protein pro-ghrelin that inhibit food intake, body weight gain and gastric emptying. The aim of the present study was to assess the serum level of obestatin and its relationship with insulin resistance in the PCOs women with normal BMI.Methods: This research is a cross-sectional study which has done on 30 PCOs patients refereed to Abolfazal Clinic. They had normal BMI diagnosed according to the Rotterdam 2003 criteria. Also, 30 women who matched regarding age and BMI with cases were selected as healthy control group. Blood samples were obtained in the follicular phase. Biochemical parameters were measured by auto-analyzer. LDL levels were calculated by using the Friedewald formula. Serum levels of insulin, obestatin, FSH, LH, TSH and Prolactin were measured by ELIZA technique with reliable kites. Result: There was no significant difference in demographic characteristics, serum concentration of lipids parameters, obestatin, insulin, TSH and FSH between cases and control groups. A significant increase was observed in FBS, HOMA.IR, LH and Prolactin in the case group compared with the control group. A reverse relationship was observed between serum level of obestatin and HOMA.IR, Insulin level and FBS.Conclusion: Lack of significant difference of obestatin concentration in PCOs compared with control group can probably be attributed to the normal BMI.

Keywords:

insulin resistant , normal BMI , Obeststin , Polycystic ovarian syndrome