Study of The Effect of Acid Amine Transfer of Arginine With Tryptophan on Polymorphism Arg194Trp in The XRC1 Gene During The Process of Spermatogenesis in Mouse Models

Today, infertility is considered as the main problem in 10-15% of people. In the meantime, idiopathic infertility (infertility with unknown causes) is widely discussed due to the wideranging point of view. In men, abnormal forms of sperm, low numbers and the absence of sperm in semen are the causes of infertility. As we know, sperm production is a complex process in which many genes are responsible for controlling each of these stages (spermatogenesis), so that we can conclude The normal performance of adult sperm depends on the structure of a DNA cloth One of the most effective genes in the spermatogenesis process is the gene XRCC1. Creating a mutation and polymorphism in this gene leads to the displacement of acid amine arginine with tryptophan, which leads to morphological and morphological disorders in the sperm and causes the inability of the sperm to perform the fertilization process. In this study to study polymorphism Arg194Trp in the XRC1 gene that results in the displacement of arginine amino acid with tryptophanIt is necessary to perform spermatic analysis in two groups of idiopathic infertile mouse in the Andrology Laboratory, which has an abnormal morphologic spermatozoicity and mouse with a non-defective fertility period In order to do modeling to prove a hypothetical precursor beginning with polymorphism in the desired design and Perform the RFLP-PCR many parts of the gene desired nucleotide Can be reproduced That can be used to identify sperm surface acid amine and studied by Elisa test.

Keywords:

Polymorphism Arg194Trp , Arginine Acid Amine , Andrology , Sperm Morphology