The study of Insulin and PMSG on Ovary in vitro and in vivo

The objective of this study was to determine the actions of different concentrations of insulin alone or in association with Pregnant Mare Serum gonadotropin (PMSG) in organ culture and evaluated follicles growth ,granulosa cells diameter and mRNA expression of cytochrome P450 aromatase (CYP19A1). Ovaries were cultured for 5 days in the absence or presence of insulin (0.2, 1, 5 and 10 μg/ml) alone or plus PMSG (10 ng/ml). In the medium with insulin 0.2 and 1 μg/ml in combination PMSG, follicles size (P<0.01), granulosa cells diameter (P<0.05) and levels of p450 aromatase (P<0.05) were significantly increased compared with other treatments. While in high dose of insulin (5, 10 μg/ml) alone or in combination with PMSG percentage of morphologically normal follicles decreased during 5 days of in vitro culture (P<0.05). In conclusion, 0.2 and 1 μg/ml insulin plus PMSG was more efficient in stimulating .follicular development and increasing expression of the CYP19A1 genes.

Keywords:

Insulin , PMSG , Organ Culture , Granulosoa Cells , Cytochrome P450 Aromatase (CYP19A1)