The effect of endurance training on dynein motor protein expression in Wistar male rats sciatic nerves with diabetic neuropathy
Publish place: Hormozgan Medical Journal، Vol: 21، Issue: 1
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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JR_HMJ-21-1_002
تاریخ نمایه سازی: 27 مرداد 1397
Abstract:
Introduction: Most neurodegenerative diseases are associated with the disruption ofaxonal transport and this might also be related to diabetes-associated disorders affectingthe nervous system. Cytoplasmic dynein is a very important motor driving the movementof a wide range of cargoes toward the minus ends of microtubules. The effects ofendurance training on dynein motor protein expression in Wistar male rats sciatic nerveswith diabetic neuropathy were investigated.Methods: Twenty one male Wistar rats were divided into three groups: diabetic control(DC: n=7), diabetic trained (DT: n=7) and Healthy control (HC: n=7). Two weeks postSTZ injection (45 mg/kg, i.p.), rats were subjected to treadmill exercise for 5 days a weekfor over 6 weeks. Levels of mRNA and protein content were determined by Real time-PCR and ELISA.Results: The blood glucose levels and dynein mRNA in both sensory and motor partsand dynein content in sciatic nerves were increased by diabetes (P≤0.05). Furthermore,exercise training modulated the dynein mRNA and dynein content to normal levels indiabetic trained rats (P≤0.05). Moreover, exercise training reduced blood glucose levelsin the DT rats (P≤0.05).Conclusion: The results of the present study confirm that dynein expresses up-regulationin STZ-induced diabetic rats. Increase in the amount of dynein expression can be acompensatory response in the lower trophic support and burden imposed on autophagysystem. These alterations suggest that retrograde axonal transport may be impaired indiabetic rats. In addition, endurance training as a non-pharmacotherapy strategy canmodulate these alterations.
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Authors
Ali Khanzani
Department of Sport Sciences, Faculty of Humanities, Young Researchers and Elite Club, Ardabil Branch, Islamic Azad University, Ardabil,Iran. Department of Physical Education and Sport Sciences
Reza Gharakhanlou
Faculty of Humanities, Tarbiat Modares University, Tehran, Iran. Department of Physical Education and Sport Sciences
Mohamad Reza Kordi
Faculty of Humanities, Tehran University, Tehran, Iran. Department of Anatomical Sciences
Mansoureh Movahedin
Facultyof Medical Sciences, Tarbiat Modares University, Tehran, Iran. Department of Physical Education and Sport Sciences