The effect of endurance training on dynein motor protein expression in Wistar male rats sciatic nerves with diabetic neuropathy

Introduction: Most neurodegenerative diseases are associated with the disruption ofaxonal transport and this might also be related to diabetes-associated disorders affectingthe nervous system. Cytoplasmic dynein is a very important motor driving the movementof a wide range of cargoes toward the minus ends of microtubules. The effects ofendurance training on dynein motor protein expression in Wistar male rats sciatic nerveswith diabetic neuropathy were investigated.Methods: Twenty one male Wistar rats were divided into three groups: diabetic control(DC: n=7), diabetic trained (DT: n=7) and Healthy control (HC: n=7). Two weeks postSTZ injection (45 mg/kg, i.p.), rats were subjected to treadmill exercise for 5 days a weekfor over 6 weeks. Levels of mRNA and protein content were determined by Real time-PCR and ELISA.Results: The blood glucose levels and dynein mRNA in both sensory and motor partsand dynein content in sciatic nerves were increased by diabetes (P≤0.05). Furthermore,exercise training modulated the dynein mRNA and dynein content to normal levels indiabetic trained rats (P≤0.05). Moreover, exercise training reduced blood glucose levelsin the DT rats (P≤0.05).Conclusion: The results of the present study confirm that dynein expresses up-regulationin STZ-induced diabetic rats. Increase in the amount of dynein expression can be acompensatory response in the lower trophic support and burden imposed on autophagysystem. These alterations suggest that retrograde axonal transport may be impaired indiabetic rats. In addition, endurance training as a non-pharmacotherapy strategy canmodulate these alterations.

Keywords:

Diabetic Neuropathy , Motor Proteins , Dynein Motor Proteins , Gene Espression