The effect of buffering leasing on parameters, chromatin and human sperm DNA

Background: Azoospermia may have a low sperm count during testicular biopsy, which is an invasive surgery. Finding this low number of sperm between blood cells is very important and by removing the blood cells, access to these sperms and their use during microinjection is easier. For this purpose, fluoridation that is used to remove blood cells should not affect sperm.Objective: This study investigates the effects of this leasing buffer on sperm parameters, sperm survival, sperm morphology, chromatin, and sperm DNA.Materials and Methods: A total of 23 samples of semen were collected and the amount of protamine deficiency was determined by staining CMA3 (chromosomal A3), the aniline blue level (AB), for anesthetic anesthetics, Toluidine blue (TB) chromatin anomalies and apoptosis with Tunnel (TUNEL) was evaluated and compared.Results: As a results, there was no significant difference in the concentration of spermatogenesis mixed with beef rhealing compared to the pre-freezer group. Positive Tunnel sperms (TUNEL +) increased significantly in the spermatozoa that had been mixed with bifurylation compared to the other group.Conclusion: Fluoridation does not affect the morphology and the low levels of prothromatin, reduces sperm motility and increases the level of chromosomal anomalies apoptozone.

Keywords:

Lising buffer , Testicular sampling , Sperm morphology , Male infertility , Sperm chromatin , Apoptosis