Association of dietary fat intake with Toll Like Receptor2 and Myeloid differentiation 88 genes expression in prepheral blood mononuclear cells and serum level of interlukin 1β in patients with metabolic syndrome

Background: Metabolic syndrome (MetS) is a proinflammatory condition that is associated with a range of metabolic disorders including glucose intolerance, abdominal obesity, dyslipidemia, and hypertension. Toll-like receptor 2 (TLR2) is a membrane immune receptor via activating cytoplasmic adapter proteins such as Myeloid differentiation 88 (Myd88) results in the production of inflammatory cytokines such as IL1β. Regarding the significant roles of diet in MetS as well as overexpression of TLR2 and myd88 in the development of obesity and inflammation, the present case-control study was aimed to investigate the association of the expression levels of TLR2 and Myd88 in PBMCs and serum levels of IL1β with dietary fat intake in Mets patients, compared to apparently healthy subjects. Materials and Methods: In this age- and gender-matched case-control study, 70 abdominally obese subjects with waist circumference (WC) ≥95cm (34 patients with MetS and 36 controls) were recruited. 5 mL blood was collected to measure serum levels of metabolic parameters and IL1β. Those with 3 or more metabolic disorders were defined as MetS, while those having 2 or less were classified as apparently healthy subjects. Also, mRNA was isolated from PBMCs and real time-PCR was performed for TLR2 as well as Myd88 genes expression assay. A 3-day food record was obtained to assay dietary fat intake. The Independent Samples t-test, Mann–Whitney U test, Chi-square test, Pearson/ Spearman or partial correlation tests were performed to assess the objectives. Results: There was a significant difference in FBS (p=0.122), TG (p<0.001), and HDL-C (p=0.005) between the two groups. There was no significant difference in the expression level of TLR2 and myd88 between the two groups; however, there was a significant correlation between the two genes in the case group (r=0.40, p=0.001). Also, there was no significant correlation between TLR2 and Myd88 genes expression level as well as serum IL1β and dietary fat intake. Conclusion: It seems that waist circumference is a chief mediator of the association of TLR2 and Myd88 as well as serum IL1β with metabolic state.

Keywords:

Toll like receptor 2 , Myeloid differentiation factor 88 , Abdominal obesity , Metabolic syndrome , Interleukin1β , Dietary fat.