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Paper
Title

In vitro and in vivo expression of virulence genes in Trueperella pyogenes based on a mouse model

Year: 1398
COI: MEDISM20_293
Language: EnglishView: 245
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

Authors

Iradj Ashrafi Tamai - Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran
Taghi Zahraei Salehi - Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abdolmajid Mohammadzadeh - Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran
Pezhman Mahmoodi - Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran

Abstract:

Introduction and Objectives: Trueperella pyogenes is an important opportunistic pathogen causing a number of pyogenic infections in ruminants and other animals. This microorganism expresses several extracellular virulence proteins that contribute to its pathogenic potential. Co-infection with other bacterial species such as Escherichia coli or Fusobacterium necrofurom increases the persistence of bacteria and the severity of the diseases. The aim of this study was expression of some pathogenesis genes including plo, nanH, fimA and cbpA and co-culturing of S. dysgalactiea, E. coli, S. aureus, F. necrophorum and L. plantarum in experimental and mice models in clinical samples collected from cattle with metritis, mastitis and skin abscess symptoms. Materials and Methods: Nine isolates with outstanding clinical symptoms including 3 metritis, 3 mastitis and 3 cutaneous abscess isolates with all virulence encoding genes were separated and cultured in TSB broth for 48 hours reaching 0.5 × 108 bacterial count forwarding RNA extraction. In the next step, co-culturing of S. dysgalactiae, E. coli, S. aureus, F. necrophorum and L. plantarum strains for 48 hours reaching 0.5 × 108 bacterial count forwarding RNA extraction were done separately to the T. pyogenes isolates. 0.5 × 108 CFU/mL of T. pyogenes co-culturing with S. dysgalactiae, E. coli, S. aureus, F. necrophorum and L. plantarum strains was formulated, injected and 48-hour intraperitoneal incubated by 0.5-mL volume into the mice categorized in 6 separately groups. So, after anesthesia and intraperitoneal cutting side of the experimental mice, liver, heart, spleen and intraperitoneal fluid of mice were collected in sterilized containers following tissue homogenization and RNA extraction according to the Kit manufacturer instruction. Results: By Livac formula, plo, NanH, cbpA and fimA genes expression observed 17, 8, 15 and 16 times more in metritis, mastitis and cutaneous abscess samples respectively. Change in plo, nanH, fimA and cbpA genes expression in co-culture in comparison with pure-culture of T. pyogenes in mice model indicated that, E. coli and F. necrophorum lead to increase and L. plantarum contributes to decrease in genes expression. There is not any significant increase observed in genes expression in co-culturing of T. pyogenes with S. dysgalactiae and S. aureus. Co-operative functions of this bacterium with other pathogens leads to enhance the expression of pathogenesis gene consequently increasing in the symptoms of the disease. Conclusion: Antagonistic effect of using of some functional bacteria and their metabolites can be very helpful for declination in expression of virulence encoding genes and pathogenesis of mentioned disease bacterial agents.

Keywords:

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This Paper COI Code is MEDISM20_293. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/987409/

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If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Ashrafi Tamai, Iradj and Zahraei Salehi, Taghi and Mohammadzadeh, Abdolmajid and Mahmoodi, Pezhman,1398,In vitro and in vivo expression of virulence genes in Trueperella pyogenes based on a mouse model,20th International Congress of Microbiology of Iran,Kerman,https://civilica.com/doc/987409

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Type of center: دانشگاه دولتی
Paper count: 11,186
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