Effect of Acute Interval Walking with Blood Flow Restriction on 4EBP1, ERK, p38, and Myostatin of SkeletalMuscle in Inactive Men

Background: There is mounting evidence that moderate‑ to high‑intensity exercise training has a key role in skeletal muscle adaption.Low‑intensity exercise with Blood flow restriction (BFR) associated with unique effect on muscle hypertrophy. Aims: The aim of this studywas to investigate the effect of acute interval walking with BFR on phosphorylation of 4EBP1, P38, ERK, and myostatin (MSTN) of skeletalmuscle in inactive men. Materials and Methods: Five healthy inactive men were participated in the study. Training protocol includesfive intervals 2‑min walking with BFR at 50%–60% maximum heart rate and 1 min at rest. All samples were collected immediately beforeexercise and 3 h after BFR training. Phosphorylation of 4EBP1, P38, and ERK skeletal muscle was evaluated by Western blotting and MSTNby Elayza test. Dependent t‑test was used to analyze the data after subtracting the posttest score from the pretest. Results: However, therewas a significant difference between the pre‑ and post‑test for 4EBP1 (P = 0.001), ERK (P = 0.049), and MSTN (P = 0.0009). There was nosignificant difference between the pre‑ and post‑test of P38 (P = 0/452) (P ≥ 0.05). Conclusion: As a result, acute interval walking with BFRactivates mammalian target of rapamycin and mitogen‑activated protein kinase pathways signaling in inactive men.

Effect of Acute Interval Walking with Blood Flow Restriction on 4EBP1, ERK, p38, and Myostatin of SkeletalMuscle in Inactive Men Keywords:

Acute interval waking , blood flow restriction , hypertrophy , phosphorylation