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Exosomes derived from ovarian epithelial carcinoma cells regulate angiogenesis by modification of jak/stat3 pathway and SOCS5 expression

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Year: 2018
COI code: BIOCONF20_244
Paper Language: English

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Authors Exosomes derived from ovarian epithelial carcinoma cells regulate angiogenesis by modification of jak/stat3 pathway and SOCS5 expression

  Fahimeh-Sadat Norouzi, - Department of Molecular Genetics, Faculty of Biological Sciences,Tarbiat Modares University, Tehran, Iran
  Mehrdad Behmanesh, - Department of Molecular Genetics, Faculty of Biological Sciences,Tarbiat Modares University, Tehran, Iran
  Sadegh Babashah - Department of Molecular Genetics, Faculty of Biological Sciences,Tarbiat Modares University, Tehran, Iran

Abstract:

Because ovarian cancer is a most fetal cancer among woman. Exosomes are intercellular carriers that are responsible for cellular communication in the tumor microenvironment they contain different materials such as miRNAs that can change the host cell s settings. jak/stat3 signaling pathway known as an important regulator of angiogenesis is inhibited by SOCS5. The purpose of this study is to determine whether ovarian-derived exosome can reduce the expression of socs5 in endothelial cells. And this decrease will increase the jak/stat3 signaling pathway and enhance the rate of angiogenesis. The exosomes were extracted from the SKOV3 cells medium by using ExoQuick kits. SEM and DLS were used to investigate in terms of size and morphology of exosomes. HUVECs were treated with exosomes (100 μg/ml) or vehicle control (PBS) for 24 and 48 hours were investigated. The PKH test was used to confirm the exosome absorption by the cell. The expression of the genes (jak1, stat3, SOCS5) was measured by qRT-PCR. SEM of purified exosome was displayed spherical and membraneencapsulated particles with diameters ranging from 50 to 200 nm. a single bell-shaped size distribution with a peak at ~90 nm resulted from DLS. Also, exosome absorption was confirmed by Fluorescent microscope and PKH assay. The results showed that exosome derived from ovarian cancer can accelerate the formation of blood vessels, reducing the expression of SOCS5 inhibitor can accelerate the expression of the jack / stat3 pathways genes and rate of angiogenesis.

Keywords:

Exosomes, Jak/stat3, SOCS5, Angiogenesis

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COI code: BIOCONF20_244

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Norouzi,, Fahimeh-Sadat; Mehrdad Behmanesh, & Sadegh Babashah, 2018, Exosomes derived from ovarian epithelial carcinoma cells regulate angiogenesis by modification of jak/stat3 pathway and SOCS5 expression, 20th National and 8th International Congress on Biology of Iran, مراغه, دانشگاه مراغه -انجمن زيست شناسي ايران, https://www.civilica.com/Paper-BIOCONF20-BIOCONF20_244.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Norouzi,, Fahimeh-Sadat; Mehrdad Behmanesh, & Sadegh Babashah, 2018)
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Type: state university
Paper No.: 25985
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