Comparsion between Synergistic Antibacterial Activities to Ag/ZnO Nanocomposites than ZnO and Ag Nano-crystales Monometallic

With the appearance of microbial organisms resistant to multiple antibiotics, also, increase nosocomial infection, antibacterial effects of nanocomposites have attended by the many researchers in resent years. In the present work, we report the synthesis of binary nano metal oxides such as Zinc Oxide/Silver, also ZnO and Ag nanocrystales monometallic via novel dry synthetic methods such as thermal decomposition of oxalate precursor. Methods: XRD, FTIR, SEM and TEM were used to the thermal spectroscopic, structural, morphological characterization and surface area determination of the product respectively. Bacterial sensitivity to nano-crystales were Specially tested using by disc diffusion test and agar dilution test, also with, determination of serial dilution test, such as; MIC, and MBC, against Escherichia coli, Salmonella galinarium, Staphylococcus aureus, Pesudomonas aueroginosa, and Bacillus subtilis, for the first time. Results: The parAcle size were 8–25 nm, approximately. The diameter inhibitory zone showed that all of the strains, have got more Sensitivity against ZnO/Ag and ZnO. Antibacterial activity of Ag nano-crystales were weaker than ZnO and ZnO/Ag. furthermore, we reported that the least value of MIC, against all strains, dependent to the ZnO/Ag NCs, wheras the great value of MIC dependent to the Ag NCs. Also, the great value of bactericidal effects displayed toAg/ZnO nanocomposites. Conclusion: This study shows that synthesise of Ag, ZnO and ZnO/Ag nanocrystales with oxalate decompositionmethod is simple and so useful. We confirmed that the antibacterial activity of ZnO nanocrystales were more vigorous than Ag nanocrystales, to all of the strains; Moreover, combination of zinc oxide and silver nanocrystales, increased their bactericidal effect. Acknowledgements: We are indebted to research Vice Chancellor of Islamic Azad University Ardabil Branch, for supporting this research.

Keywords:

Ag/ZnO nanocomposites , Synergistic antibacterial activities , Thermal Decomposition of Oxalate Precursor Method