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The Relationship between the Expression of Small Heat-Shock Proteins and Suppression of OCT4B1

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Year: 2019
COI code: SRCCG05_041
Paper Language: English

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Authors The Relationship between the Expression of Small Heat-Shock Proteins and Suppression of OCT4B1

  Mohammad Reza Mirzaei - Assistant Professor of Molecular Genetics, Molecular Medicine Research Center, ResearchInstitute of Basic Medical Sciences and Department of Clinical Biochemistry Rafsanjan University of Medical Sciences, Rafsanjan, Iran
  Shima Khajouee Ravari - MSc of Molecular Genetics, Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
  Gholamhosein Hasanshahi - Professor of Hematology, Molecular Medicine Research Center, Research Institute of Basic Medical Sciences and Department of Immunology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
  Mehdi Mahmoodi - Professor of Clinical Biochemistry, Molecular Medicine Research Center, ResearchInstitute of Basic Medical Sciences and Department of Clinical Biochemistry Rafsanjan University of Medical Sciences, Rafsanjan, Iran
  Rahim Heidari barchi nezhad - MSc of Immunology, Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
  Fatemeh asadi - MSc of Biochemistry, Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Abstract:

Background: OCT4B1, a variant of OCT4, is expressed in both cancer cells and tissues. This variant has a main role in the regulation of both apoptotic and stress heat-shock proteins pathways. The aim of this study was to investigate the effects of OCT4B1 silencing on the expression of small heat-shock proteins sHSPs in three human tumor cell lines.Methods: AGS gastric adenocarcinoma , 5637 bladder tumor and U-87MG brain tumor cell lines were transfected with specific OCT4B1 siRNA test group and scramble siRNA control group , using siRNA and Lipofectamine. Real-Time PCR Array technique was applied and the fold changes were calculated using RT2 Profiler PCR Array Data Analysis version 3.5.Results: It was revealed that HSPB1, HSPB6 and HSPE1 were down-regulated in all three studied tumor cell lines and HSPB2, HSPB7 and HSPB8 were down-regulated in two of three studied tumor cell lines AGS and 5637 . It was also revealed that HSPB3 was down-regulated in 5637 cell line and up-regulated in AGS and U87MG cell lines.Conclusion: According to the results, it may be concluded that there is a direct relationship between OCT4B1 and sHSPs gene family expression. Thus, suppression of OCT4B1 may be considered in cancer therapy/research.

Keywords:

OCT4B1, Small HSPs, Tumor cell lines

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COI code: SRCCG05_041

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Mirzaei, Mohammad Reza; Shima Khajouee Ravari; Gholamhosein Hasanshahi; Mehdi Mahmoodi; Rahim Heidari barchi nezhad & Fatemeh asadi, 2019, The Relationship between the Expression of Small Heat-Shock Proteins and Suppression of OCT4B1, 5th congress of student research committee of rafsanjan university of medical sciences, رفسنجان, دانشگاه علوم پزشكي رفسنجان, https://www.civilica.com/Paper-SRCCG05-SRCCG05_041.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Mirzaei, Mohammad Reza; Shima Khajouee Ravari; Gholamhosein Hasanshahi; Mehdi Mahmoodi; Rahim Heidari barchi nezhad & Fatemeh asadi, 2019)
Second and more: (Mirzaei; Khajouee Ravari; Hasanshahi; Mahmoodi; Heidari barchi nezhad & asadi, 2019)
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Type: Medical University
Paper No.: 288
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