Synthesis of 1,4-dihydropyridine derivatives in the presence of MNPs-trypsin as a biocatalyst.

eworthy1,4-Dihydropyridine (1,4-DHP) ring is a challenging structural core in organic chemistry due to its biological properties, especially as calcium channel blockers. It isnoteworthy that its range of application has been recently extended to other affections such as antioxidant, antidiabetic and antitumor agents.1 A rapid, efficient and eco-friendly protocol has been developed for the preparation of various 1,4-dihydropyridine derivatives in the presence of biocatalyst. The process presented here is operationally simple, environmentally benign and has excellent yield. Furthermore, the biocatalyst can be recovered conveniently and reused efficiently.2 For preparing the MNPs-trypsin biocatalyst, we first synthesized Fe3O4 magnetic nanoparticles (MNPs). Trypsin was then immobilized on the magnetic coreshell particles by using glutaraldehyde as a cross-linker.3 We used this synthesized MNPstrypsin biocatalyst in the reaction of 1,4-DHP and the results were amazing. Moreover, the 1H and 13C chemical shift and X-ray spectra values together with the structural parameters of 1,4- dihydropyridine have been computed and the scaled values have been compared with the corresponding experimental NMR and X-ray spectra. As a result, the calculated 1H and 13C chemical shift and X-ray spectra show a good agreement with the experimental results.

Keywords:

1 , 4-Dihydropyridine , MNPs-trypsin biocatalyst , Green chemistry , Enzyme