Investigation of Total flavonoid of ۵۵ Iranian Berberis genotypes

The genus Berberis belongs to the Berberidaceae family, with ۱۵ genera and ۶۵۰ species around the world. It has valuable potential in the medicinal and food industries. In this study, the Total flavonoid of ۵۵ fruits of the Iranian Berberis genotype were investigated. The results of this study could be used in the breeding and determination of superior genotypes in the future. Plant materials were collected from the barberry Collection Garden of Mashhad and also different natural habitats of barberry in various provinces of Iran. The samples were air-dried, finely grounded, and extracted by methanol at room temperature. Then, total flavonoid was measured by AlCl۳ assays. The results showed a significant difference between flavonoid content of various extracts, at a probability level of ۱%. The highest flavonoid content was observed with extracts of B. integerrima (genotype code ۴-۴) and B. orthobotrys × crataegina (genotype code ۱۵-۴) with an average of ۶.۳ mg quercetin equivalents (Q) ml-۱ extract. The least was recorded for B. integerrima (genotype code ۲۳-۴) with an average of ۰.۴ mg quercetin equivalents (Q) ml-۱ extract. The results showed that barberry has a great diversity in terms of phytochemical characteristics in different genotypes and is a valuable genetic source for breeding research.

Keywords:

Total Flavonoid , AlCl۳ assays , Berberis