Antioxidant, enzyme activity and antifungal properties of Satureja khuzistanica treated by multi-walled carbon nanotubes

Satureja khuzistanica is a medicinal herb from the Lamiaceae family that is endemic to Iran. It is known for its antifungal and antioxidant compounds, especially rosmarinic acid (RA). The study examines the time course impacts of multi-walled carbon nanotubes (MWCNTs) on RA production and the phytochemical and antifungal properties of S. khuzistanica. In vivo, plants were time-course treated with MWCNTs. RA was measured by HPLC. Catalase (CAT), guaiacol peroxidase (POD), and ascorbate peroxidase (APX) were quantified. DPPH and β-carotene were also assayed. The antifungal effects of samples were evaluated against Fusarium solani K (FsK). The highest RA production was achieved at ۲۴ h MWCNTs. The highest CAT, POD, and APX activities were also observed at ۲۴ h MWCNTs. DPPH and β-carotene showed ۵۰% and ۸۰% inhibition at ۴۸ h of treatment, respectively. The FsK aggregation was the lowest for callus in number of conidia, fresh weight, and dry weight that proved RA inhibitory effects. The callus reduces the FsK growth diameter to ۲.۷۵ on the ۵th day. The use of MWCNTSs increased RA in S. khuzistanica and revealed potential pharmaceutical and antifungal properties

Keywords:

Satureja khuzistanica , multi-walled carbon nanotubes , rosmarinic acid , Fusarium solani K