The Significant Association of the dupA and cagA genes of Helicobacter pylori with Peptic Ulcer

Background: Helicobacter pylori play a significant etiological role in various digestive diseases. Peptic ulcer is caused by H. pylori, which destroys the duodenum mucus and is often observed in the individuals consuming tobacco, spicy and heavy meals, alcohol, coffee, and tranquilizers. Several studies have indicated that duodenal ulcer promoting genes dupA and cagA are involved in H. pylori etiology. The present study aimed to evaluate the correlation between these genes and peptic ulcer.  Methods: In this study, ۵۰۰ stomach biopsy samples were assessed based on the rapid urease test and polymerase chain reaction (PCR) for H. pylori infection, followed by histological and microscopic examinations. Results: The dupA and cagA genes were subjected to PCR. Although dupA showed no significant correlation with peptic ulcer, the cagA genotype had a significant association with peptic ulcer (P < ۰.۰۵). Similar to the dupA gene, blood group was not observed to be correlated with H. pylori infection. Conclusion: According to the results, there are significant correlations between tobacco use (P < ۰.۰۵), tranquilizer use (P < ۰.۰۵), and meteorism (P < ۰.۰۵) with peptic ulcer. In addition, the expression of the cagA and dupA genes was investigated in patients with non-ulcer dyspepsia and peptic ulcer.

Keywords:

Helicobacter pylori , Peptic ulcer , Real time technique , dupA , cag-A