Study of cyp۲E۱ gene RsaI/PstI polymorphisms in patients with gastric cancer in north of Iran

Background: North of Iran is amongst high incidence rate areas of gastric carcinoma where environmental carcinogenic compounds especially agricultural pesticides are massively used. Cytochrome P۴۵۰ ۲E۱ (CYP۲E۱) enzyme metabolically activates a large number of low molecular mass xenobiotics. The polymorphic nature of cyp۲E۱ gene control elements is associated with interindividual differences for toxicity of its substrates and may be responsible for increased gastric cancer susceptibility. The current study investigated the allelic frequencies of cyp۲E۱ gene RsaI/PstI polymorphisms and its association with gastric cancer risk in north of Iran. Materials and Methods: This case-control study comprised of ۱۲۰ gastric cancer patients and a group of ۱۳۵ healthy individuals as control. Genotyping of cyp۲E۱ gene PstI/RsaI polymorphisms were carried out by PCR-RFLP method. Statistical analyzes were performed by Logistic regression model and P<۰.۰۵ was considered as significance level. Results: TNM classification showed that most patients (۸۸%) were in advanced stages when the disease was diagnosed. Frequencies of C۱C۱ and C۱C۲ genotypes of PstI/RsaI polymorphisms were ۹۶ and ۴% in case, and ۹۹ and ۱% in control group, respectively whereas homozygote C۲C۲ genotype was not observed in any of the subjects. In Logistic regression model no significant association was found between RsaI/PstI allelic variants and gastric cancer risk (p =۰.۴۴۳, OR=۰.۳۸۶, CI=۰.۰۳۴-۴.۳۹۵). Furthermore, no significant correlation was seen between genotypic frequencies and clinicopathological characteristics. Conclusions: No significant association was found between cyp۲E۱ gene PstI/RsaI allelic variants and gastric cancer risk or clinicopathological characteristics of gastric cancer patients in north of Iran.

Keywords:

cyp۲E۱ gene , Gastric Cancer , PstI/RsaI polymorphism , Northern Iran.