Evaluation of Phenolic and Flavonoid Content, Alkaloids, Antioxidant Capacity and Antibacterial Properties of Methanolic Extract of Zahak Native Medicinal Plants Against Seven Pathogens

Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JJMPB-13-1_007

تاریخ نمایه سازی: 8 بهمن 1402

Abstract:

In general, the resistance of microorganisms to antibiotics has become one of the major concerns of human society and the health care system. The aim of this study was to evaluate total phenol, flavonoids content, alkaloids, antioxidant capacity and antibacterial activity of ۷ medicinal plants extracts in Sistan climatic conditions against pathogens. Plant samples were collected from the collection of the Agricultural Research Institute of medicinal plants, University of Zabol and dried in normal shade and room temperature. A Methanolic extract of the leaves of snake grass, eucalyptus, tatura, Musquit bean, Watercress, rosemary and olive was prepared by cold maceration method. Total phenol content by using folin-ciocalteu reagent method, total flavonoid content by aluminum chloride colorimetric method, antioxidant capacity by DPPH free radical scavenging assay and antibacterial activity of extracts by agar diffusion method and measurement of growth inhibition zone or Disk diffusion was measured. The analysis of variance showed that there was a statistically significant difference between different medicinal plants at a probability level of ۵%. In the present experiment, the highest phenolic content (۱۱۰.۷۸ mg/q D.W.) in the Rosemary methanolic extract, the highest total flavonoid content (۴.۵۵ mg GA/g D.W.) in Eucalyptus extract and the highest antioxidant activity (۹۳.۱%) in the Olive extract were observed. Also, the largest diameter of Disk diffusion (۲۵ mm) was observed in the medium containing Rosemary extract and against Streptococcus pyogenes.

Authors

Bahman Fazeli-Nasab

Department of Agronomy and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol, Iran

Mehrangiz Ghafari

Department of Pathology, School of Medicine, Zabol University of medical sciences, Zabol, Iran

Mehdi Jahantigh

Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

Zahra Beigomi

Zahedan University of Medical Sciences, Zahedan, Iran

Saeide Saeidi

Agricultural Biotechnology Research Institute, University of Zabol, Zabol, Iran

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