The Effects of Limonene and Orange Peel Extracts on Some Spoilage Fungi

Orange peel extract solution contains mucilage flavor and three types of glycosides. Limonene is one of the terpenoides in some vegetables, fruits and food; that plays as an antioxidant in juice. As it has anti-microorganism effects on some bacteria, we carried out the antifungal effects of limonene versus orange extract. In the present study the effects of orange extract and two types of limonene against Candida albicans, Aspergillus niger, Aspergillus sp. and Penicillium sp. has been studied using agar well diffusion and paper disc diffusion. (S)-(-)-Limonene had more inhibitory effect on examined fungi; in both well diffusion, and paper disc diffusion experiments. Aspergillus niger was more sensitive related other fungi. Orange peel extract didn’t show any antifungal activity on tested fungi. The results showed that orange peel extract does not have any antifungal activity. However Limonene (S)-(-) type has been shown to have inhibitory effects on filamentous fungi.

Keywords:

Limonene , antifungal effect , Orange peel extract , paper disc diffusion , Agar well diffusion