Evaluation of the Recombinant Escherichia coli BL21(DE3) Growth Kinetics in Glucose-limited Chemostat Culture

The relationship between specific growth rate (µ) and steady-state glucose concentration was investigated for Escherichia coli BL21(DE3) in carbon-limited chemostat culture. Cells initially were cultivated in batch culture at high glucose concentrations and then feed was continuously added at specified flow rates to generate various dilution rates (0.1 h- l < D < 0.8 h-1). Statistical evaluation of different kinetic models showed that calculated parameters have been in various ranges. Also comparison between evaluated parameters from chemostat and batch process showed that chemostat is the foremost method for obtaining growth kinetic’s parameters.

Keywords:

Continuous culture , Kinetic models , Dilution rate , Chemostat , Recombinant , Escherichia coli