Evaluation of polyploidy induced in terms of chlorophyll content and catalase activity in Borage (Echium amoenum Glowacka)

In this work, the effect of polyploidy was evaluated through catalase activity, chlorophyll a, chlorophyll b, cartnoeid and carbohydrate. This study was used in different levels of colchicine (0, 0.25, 0.5, 0.75 and 1% w/v) in a different time period (24, 36 and 48h) in a completely randomized design with 3 replications. Cytogenetic results showed that chromosome number changed in 0.75 and 1% Colchicine concentration levels and 24, 36 and 48h time period. Analyze of variance showed that the effect of colchicine levels, time duration and interaction for all traits, except the effect of time duration for cartnoeid were significant (P < 0.01). Results showed that the highest catalase, chlorophyll a, chlorophyll b and cartnoeid were attained from 0.25% colchicine with 24 and 36 h time duration conditions. Therefore, colchicine polyploidy induction in current and other studies can be used in plant breeding to for produce superior varieties of plants.

Keywords:

Borage , Colchicine , Chlorophyll , Polyploidy , Antioxidant enzyme