Molecular approaches for detection and identification of foodbornepathogens
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JFQHC-1-1_001
تاریخ نمایه سازی: 20 آبان 1397
Abstract:
oodborne pathogens comprise microorganisms such as viruses, bacteria and parasites that can be transmitted by food and affect public health worldwide. The most common viruses transmitted via food are hepatitis A virus and Norwalk-like caliciviruses. Also, the most common bacteria involved in foodborne illnesses are Campylobacter jejuni, Clostridium perfringens, Salmonella spp, Escherichia coli O157:H7; and the most important parasites that can cause these conditions are Giardia duodenalis, Cryptosporidium parvum, Cyclospora cayetanensis, Toxoplasma gondii,Trichinella spiralis, Taenia saginata and/or solium, Entamoeba histolytica, Anisakis spp. and Diphyllobothrium spp. Because of their eventual small number in the sample, their detection and identification is not always easy. On the other hand, conventional methods like cultures are almostlabor intensive, time consuming and costly. Recently, molecular techniques have been developed for rapid, sensitive and specific identification. The most common molecular methods are polymerase chain reaction (PCR)-based techniques. In this article, the sensitive and specificmolecular tests for routine detection and identification of foodborne pathogens are reviewed.
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Authors
S Boughattas
Department of Environmental and Biological Chemistry, College of Agriculture, Life and Environmental Sciences, Chungbuk NationalUniversity, Cheongju, Chungbuk, South Korea
R Salehi
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran