Comparing The Effects of Glibenclamide/Caffeine Combination Therapy and Glibenclamide/Diazepam on Oxidative Stress of Renal Tissue in Diabetic Rats abstract
Background and Objective:Oxidative stress has a variety of destructive effects on different parts of the body such as kidney tissue. Diabetic nephropathy is among serious complications of diabetes. One of the complications of diabetic nephropathy is distention and destruction of glomeruli in the renal tissue. Glibenclamide is one of the materials widely used in diabetes, but it has a lot of adverse effects including hypoglycemic shock. Other materials widely used in various countries include caffeine and diazepam.
Caffeine has anti-oxidant effects. This study was performed to survey the effect of glibenclamide with caffeine combination therapy and glibenclamide with diazepam on oxidative stress on renal tissue in diabetic rats. Materials and Methods:In this experimental study, 48 male rats, weighing 200 - 250 g were divided into six groups: 1: diabetes mellitus (DM), 2: DM + glibenclamide, 3: DM + caffeine, 4: DM +glibenclamide + caffeine, 5: DM + diazepam, and 6: DM + glibenclamide + diazepam. Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg). After three days post-injection, diabetes was verified using glucometer. Treated group with glibenclamide, caffeine and diazepam received daily 0.3 mL, 0.5 ml and 0.2 mL, respectively. The group treated with glibenclamide and caffeine, and the group treated with glibenclamide and diazepam received daily 0.15 mL, 0.25 mL, 0.15 mL and 0.1 mL, respectively by intraperitoneal injection. In the second week after drug injection, rats were anesthetized and kidney tissue removed. Malondialdehyde (MDA) and protein were measured by Thiobarbituric and Bradford method and then evaluated by spectrophotometery. Quantitative data was analyzed by one-way ANOVA using SPSS. Results:Statistical analysis results showed that using glibenclamide (P=0.009), caffeine (P=0.009) or diazepam (P=0.008) alone, and also combination therapy of glibenclamide with caffeine (P=0.008) and glibenclamide with diazepam (P=0.009), in diabetic rats decreased kidney tissue level of MDA significantly.Conclusion:As a result using diazepam or combination of glibenclamide with caffeine had better effects than using caffeine, glibenclamide, or combination of glibenclamide with diazepam, in lipid peroxidation reduction in kidney tissue of diabetic rats.