Cholesterol Biosensor Based on Immobilization of Cholesterol Oxidase on a Gold–Platinum Bimetallic Nanoparticles Modified Glassy Carbon Electrode

A novel cholesterol biosensor was constructed, based on the immobilization of cholesterol oxidase (COx) with cross-linking in the matrix of biopolymer chitosan (CS) on a glassy carbon electrode (GCE), which was modified with gold–platinum alloy nanoparticles (Au–Pt NPs) by electrodeposition in CS film. The properties of Au–Pt NPs/CS were characterized by scan electron microscopy (SEM), cyclic voltammetry (CV). Primary study indicated that Au–Pt NPs had a better synergistic electrocatalytic effect on the determination of cholesterol than did Au NPs/CS or Pt NPs/CS at a low applied potential window.

Keywords:

cholesterol , Au–Pt alloy nanoparticles , Chitosan , Electrodeposition , Biosensor