Conserved OprF as a Selective Immunogen Against Pseudomonas aeruginosa
Publish place: IRANIAN JOURNAL of PATHOLOGY، Vol: 12، Issue: 2
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJP-12-2_011
تاریخ نمایه سازی: 1 مهر 1398
Abstract:
Background & Objectives: Due to the importance of Pseudomonas aeruginosa in severe inpatient infections and high mortality, the need for an efficient vaccine against these bacteria is increasing. In this regard, the general outer membrane porin of the most problematic microorganism P. aeruginosa, outer membrane protein F (OprF), is a good vaccine candidate. Methods:The databank of NCBI was used to retrieve protein sequences recorded for OprF in P. aeruginosa.The current study aimed at investigating the conservation of the OprF in 150 reference sequences, clinical, and environmental strains of P. aeruginosa from different countries via bioinformatic tools.T-COFFEE and PRALINE software were used for alignment. Results: Of these, 134 strains were isolated from clinical specimens and other strains from environmental samples. Evaluation of alignment by the mentioned software clearly showed that this protein was conserved. Antigenicity and grand average of hydropathicity were favorable. Conclusion: Conservation of OprF in all pathogenic and environmental strains of P. aeruginosa indicated that it can be considered as a good immunogen; however, the protectivity of OprF should be validated experimentally.
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Authors
Ehsan Kazemi Moghaddam
Dept. of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.
Parviz Owlia
Molecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, Iran.
Abolfazl Jahangiri
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iraj Rasooli
Dept. of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.
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