HPV and Helicobacter Pylori infection in Head and Neck Squamous Cell Carcinoma in An Iranian Population

Background:Head and neck squamous cell carcinomas (HNSCC) are a major health issue in many parts of the world. Recently, attention has focused on infections as a potential causative agent for HNSCC. The association between the patients with HPV and H.pylori and the prevalence of these factors and the progression of the disease and the location of the lesion in this study have been investigated.Methods:In this study, 62 patients with HNSCC who referred to Mashhad University of Medical Sciences from Jan 2016 to Feb 2018 entered the study. These patients include 47 men and 15 females with mean age of 60.5±13.4 years. Fresh tumor tissue and healthy tissue surgical margins were collected. Then, DNA extraction from fresh tumor tissues and fresh healthy margin tissues. To detect H. pylori in tissue samples specific primers targeting 16S rRNA and glmM genes were used. For HPV detection, specific primers targeting MY09and MY11 genes were used.Results:Patients who presented with advanced-stage disease were 34 patients (54.8%). Grade I was the most frequent among patients (61.3%). SCC in larynx area was highest frequency in patients (41.9%). There was a significant increase in HPV infection in the advanced stage group compared to the early stage group. In our study, there was a significant relationship between stage in HNSCC patients with lymph node involvement and grade. (Pvalue<0.05)Conclusion:In our study, there was a significant relationship between HPV infection in HNSCC patients with age, stage and grade. However, there was no significant relationship between these factors in patients with h pylori virus.

Keywords:

Human Papillomavirus , Head and Neck Squamous Cell Carcinoma , Helicobacter pylori