Evaluation of NLRP۳ gene expression level in patients with coronary artery disease and its comparison with healthy Persons

NLRP۳ is one of the most important inflammatory flames, which is a set of multi-protein signaling. The NLRP۳ gene is known to be associated with many human autoimmune and inflammatory diseases.Therefore, the aim of this study was to evaluate the expression of NLRP۳ gene in patients with coronary artery disease and compare it with healthy individuals .In this study, sampling was performed on all individuals who had referred to Shariati Hospital in Tehran for diagnostic angiography of coronary artery disease within ۵ months .Patients were divided based on normality and coronary artery occlusion and evaluated for NLRP۳ expression after RNA extraction from peripheral blood and cDna fabrication by Real time PCR .All patients with and without diabetes and hypertension were also evaluated .According to the results, there was no significant difference in the expression of NLRP۳ gene in patients with coronary artery stenosis compared to patients without coronary artery stenosis and also in the presence or absence of diabetes and hypertension .However, various studies have previously reported the association of the NLRP۳ gene with the disease.

Keywords:

Inflammasome , Real time PCR , Diabetes , Blood pressure , Lupus