The study of cytotoxic effects of the gum of Ferula gummosa on PC-۳ cells

The genus Ferula is composed of ۱۸۰ species, including ۱۵ species are endemic of Iran, ۹ endemic species of Turkey, ۷ Chinese endemic species and one Italian endemic Sepices and remainder grow in other countries. Ferula species are rich of different compounds including Sesquiterpene coumarins, Sesquiterpenes, Sesquiterpenes coumarin glycosides and sulfur containing compounds. According to studies, Ferula genus has different activities such as anticancer, anti-bacterial, anti-fungal, anti-epileptic and anti-oxidant. Ferula gummosa grows in different parts of Iran. In the past, it used to reduce abdominal pains, It is used as laxative and also to treat Rheumatism and diabetes. F.gummosa has a milky white gum resin extraction from the roots and stems. In this study, we investigated the cytotoxic effect of F. gummosa on PC-۳ cells. After preparation of the gum of F.gummosa, PC-۳ cells were treated with different concentrations of the gum for ۴۸ hours. The IC۵۰ value of the gum for PC-۳ cells was calculated to be ۹.۱۴μg/ml. The DNA fragmentation assay showed that۱۰ μg/ml of the gum induced apoptosis in treated cells .The result of clonogenic assay showed a reduction in cell proliferation at concentrations of ۴ and ۶ μg/ml of the gum, and treatment of cells with ۷ and ۸ μg/ml of the gum led to more reduction in cell proliferation.

Keywords:

Ferula gummosa , Cytotoxicity , Prostate cancer