The Effect of ۸ weeks of High Intensity Interval Training on Gene Expression of Some Hepatokines in Rats with Nonalcoholic Fatty Liver

Non-alcoholic fatty liver disease (NAFLD) is one of the most common forms of chronic liver disease. NAFLD is a reversible disease characterized by excessive accumulation of triacylglycerols in the liver. NAFLD ranges from hepatic steatosis to nonalcoholic steohepatitis (NASH) and liver cirrhosis. Some of these proteins, including fibroblast growth factor (FGF۲۱), follistatin, and leukocyte-derived leukocyte cells (LECT۲), are modulated by metabolic stresses.Twenty-four male Wistar rats were prepared from Pasteur Institute of Iran. The animals were divided into ۳ groups of ۸ rats: ۱- healthy control, ۲- fatty liver control, ۳- fatty liver +interval training. Except for the healthy control group, the disease was induced in the other groups. The animals in the exercise groups performed the periodic exercise protocol for eight weeks (three sessions per week, each session lasting ۱۰ minutes). One-way analysis of variance was used to determine the significant differences between the research groups and a significance level of P≥۰.۰۵ was considered.The results showed that there was no significant difference in the expression of follistatin and LECT۲ gene in the study groups, although the relative expression of FGF-۲۱ gene in the fatty liver control group was higher than the healthy control, but it was not statistically significant.Based on the findings of this study, High Intensity Interval Training has no effect on the expression of hepatokines genes follistatin, FGF۲۱ and LECT۲. Further research is needed to better understand the interaction between these proteins as well as their interaction with exercise and other metabolic disorders.

Keywords:

LECT۲ , FGF۲۱ , FOLLISTATIN , HIIT , NAFLD